Structural Basis of Transcription Activation: The CAP- CTD-DNA Complex
نویسندگان
چکیده
The Escherichia coli catabolite activator protein (CAP) activates transcription at Plac, Pgal, and other promoters through interactions with the RNA polymerase subunit carboxyl-terminal domain ( CTD). We determined the crystal structure of the CAPCTD-DNA complex at a resolution of 3.1 angstroms. CAP makes direct protein-protein interactions with CTD, and CTD makes direct protein-DNA interactions with the DNA segment adjacent to the DNA site for CAP. There are no large-scale conformational changes in CAP and CTD, and the interface between CAP and CTD is small. These findings are consistentwith the proposal that activation involves a simple “recruitment” mechanism.
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